Journal: The Journal of Biological Chemistry

Article Title: The fates of internalized Na V 1.7 channels in sensory neurons: Retrograde cotransport with other ion channels, axon-specific recycling, and degradation

doi: 10.1016/j.jbc.2022.102816

Figure Lengend Snippet: Na V 1.7-containing endosomes are delivered to lysosomes. A , distal axons were labeled using cell-impermeable Halo-tag ligand JF635i, incubated for 6 h, fixed with paraformaldehyde, and then imaged using SRRF microscopy. The black inset shows a DRG soma, which contains both LAMP1-mNeonGreen and Halo-Na V 1.7. The red inset shows a super-resolution image of LAMP1-labeled lysosomes colocalized with Halo-Na V 1.7. B , normalized fluorescence across the dotted line in ( A ), red inset , merge, demonstrates the presence of Halo-Na V 1.7 within the lysosomal lumen. This pattern of labeling was observed in 30 cells from three independent cultures. DRG, dorsal root ganglion; LAMP1, lysosomal-associated membrane protein 1; Na V , voltage-gated sodium channel; SRRF, super-resolution radial fluctuation.

Article Snippet: SRRF microscopy was performed using the imaging system described previously together with the Andor SRRF-Stream software (Oxford Instruments).

Techniques: Labeling, Incubation, Microscopy, Fluorescence

Journal: Scientific Reports

Article Title: Multiband and multifunctional polarization converter using an asymmetric metasurface

doi: 10.1038/s41598-021-88771-x

Figure Lengend Snippet: Geometry of the proposed single layer converter, ( a ) without SRR, ( b ) with SRR (figures are extracted from CST microwave Studio software version 2018).

Article Snippet: Figure 1 Geometry of the proposed single layer converter, ( a ) without SRR, ( b ) with SRR (figures are extracted from CST microwave Studio software version 2018).

Techniques: Software